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iCell Microglia, 01279
Human iPSC-derived microglia from a Caucasian male (age 50-59) with a healthy phenotype, differentiated from blood-derived iPSCs.
Products
Kits
- 1 vial Cryopreserved iCell GFP Microglia, 01279
- 50ml iCell Glial Base Medium
- 0.5ml iCell Microglia Supplement A (100x)
- 0.5ml iCell Microglia Supplement B (100x)
- 1ml iCell Neural Supplement C (50X)
- iCell Microglia Quick Guide
- Additional reagents required; see Quick Guide
genetic status
Quantity (Cells Per Vials)
Catalog #
Catalog #:
Microglia differentiated from human iPS cells, frozen
From
$2,395.00
Cells Only
- 1 vial Cryopreserved iCell GFP Microglia, 01279
- iCell Microglia Quick Guide
- Additional reagents required; see Quick Guide
genetic status
Quantity (Cells Per Vials)
Catalog #
Catalog #:
Microglia differentiated from human iPS cells, frozen
From
$2,095.00
Product Overview
Microglia are immune cells resident in the central nervous system (CNS) responsible for fundamental physiological and pathological processes. Microglia support neuronal homeostasis and facilitate neuronal network formation by synaptic pruning. Surveillant microglia can activate, adapt and respond accordingly to specific stimulus. Microglia dysfunction has been implicated in neurodegeneration afflictions, including Parkinson’s and Alzheimer’s diseases, and neurodevelopmental disorders, such as Rett syndrome. The limited availability and inconsistency of primary human microglia has constrained research and therapeutic progress.
iCell® Microglia are based on technology developed by the Blurton-Jones laboratory (Abud et al. Neuron 2017.) for which FUJIFILM Cellular Dynamics Inc. has an exclusive license from the University of California-Irvine. iCell Microglia overcome the limitations of primary microglial cells by offering a consistent, high-quality microglia derived from human induced pluripotent stem cells, enabling the study of cytokine signaling, synaptic transmission, and plasticity in normal CNS function and during disease progression.
- Human Relevance
iCell Microglia exhibit functional characteristics similar to human microglia, including phagocytosis and cytokine-mediated inflammatory responses, and express relevant microglial markers (TREM2, CX3CR1, TMEM119, P2RY12, IBA1). - Reproducible Research
Rigorous quality control and large-scale manufacturing capabilities ensure consistent performance and reproducible results from every lot of iCell Microglia. - Rapid Results
iCell Microglia are shipped cryopreserved and arrive fully differentiated allowing more experiments to be completed in less time. - Disease Models Available
Engineered TREM2 knockout die model lines of iCell Microglia are available as well as Alzheimer’s Disease donor iCell Microglia featuring ApoE4/4 or TREM2 R47H genotypes. - Fluorescent Reporters Available
Engineered Green Fluorescent Protein (GFP) iCell Microglia are available for real-time in vitro visualization of cells and morphology.
Components:
Our regular business hours are 9:00am to 5:00pm Central Time (USA)
Need Help With This Product?
Our specialists are here to help you find the best product for your application.
Our regular business hours are 9:00am to 5:00pm Central Time (USA)
Technical Docs
PROTOCOLS
Application Protocols
Immunofluorescent Labeling of iCell Microglia
Application Protocols
Labeling Amyloid Beta with pHrodo Red
Application Protocols
Measuring Microglia Phagocytosis: Kinetic Imaging on the IncuCyte
Application Protocols
Tri-Culture of iCell Microglia with iCell Neuronal Cell Types and iCell Astrocytes
USER DOCS
Quick Guide
iCell Microglia Quick Guide
Datasheet
iCell Microglia
Biosafety Document
iCell Microglia Biosafety Document
Safety Data Sheet
iCell Neural Supplement C (50X) Safety Data Sheet - Asia
Safety Data Sheet
iCell Neural Supplement C (50X) Safety Data Sheet
Safety Data Sheet
iCell Glial Basal Medium Safety Data Sheet - Asia
Safety Data Sheet
iCell Glial Basal Medium Safety Data Sheet
Safety Data Sheet
iCell Neural Supplement C (50X) Safety Data Sheet
Safety Data Sheet
iCell Neural Supplement C (50X) Safety Data Sheet - Asia
Safety Data Sheet
iCell Cell-Based Products Safety Data Sheet - Asia
Safety Data Sheet
iCell Cell-Based Products Safety Data Sheet
Safety Data Sheet
iCell Glial Base Medium Safety Data Sheet
Safety Data Sheet
iCell Glial Base Medium Safety Data Sheet - Asia
Safety Data Sheet
iCell Neural Supplement C Safety Data Sheet
Safety Data Sheet
iCell Neural Supplement C Safety Data Sheet - Asia
Safety Data Sheet
iCell Microglia Supplement A Safety Data Sheet
Safety Data Sheet
iCell Microglia Supplement A Safety Data Sheet - Asia
Safety Data Sheet
iCell Microglia Supplement B Safety Data Sheet
Safety Data Sheet
iCell Microglia Supplement B Safety Data Sheet - Asia
Performance Data
iCell Microglia are a Highly Pure Microglial Population
Based on published data describing gene expression and signatures of several microglial stages (Abud et al, 2017a>), iCell Microglia represent a valid in vitro model for CNS research. These cells express TREM2, CXCR4, P2RY12, IBA1, and PU.1 microglial markers at over 80% in the overall cell population. Physiologically, iCell Microglia demonstrate critical responses to stimuli including signature cytokine release, and the phagocytosis of bioparticles. Flow cytometry analysis of surface markers shows that iCell Microglia exhibit a characteristic microglia signature.
Figure 1: iCell Microglia are a Highly Pure Microglial Population
Figure 2: iCell Microglia Secrete Cytokines Upon Stimulation
iCell Microglia Secrete Cytokines Upon Stimulation
iCell Microglia were thawed into 96-well plates and allowed to recover for three days prior to treatment (using lipopolysaccharide (100ng/mL) or IFN gamma (50ng/mL). After 24 hr, cytokine levels in the supernatant were assayed using the Luminex Multiplex Assay. Specific cytokine signatures were observed for each stimulus.
iCell Microglia Phagocytose Bioparticles
Using the IncuCyte® Live-Cell Analysis System (Sartorius AG), Staphylococcus aureus (SA) BioParticles® Conjugate (ThermoFisher Scientific) were added to iCell Microglia cultures to assess the kinetics of phagocytosis.
Figure 3: iCell Microglia Phagocytose Bioparticles
Product Highlights
iCell Microglia Physiology
iCell Microglia are semi-adherent cells amenable to a variety of applications. iCell Microglia express key transcription factors and markers (CX3CR1, IBA1, TMEM119 and P2RY12) that are characteristically found in adult human microglia and are able to phagocytose and secret cytokines upon stimulation. Cell-based Assays Immunofluorescence Phagocytosis Amyloid-beta pHrodo™ Red labeling
Figure 1: iCell Microglia Physiology (A) iCell Microglia secrete specific cytokines upon stimulation. (B) iCell Microglia phagocytose BioParticles® in a concentration-dependent manner.
Advantages
- Human relevance: iCell Microglia exhibit functional characteristics similar to human microglia, including phagocytosis and cytokine-mediated inflammatory responses.
- Reproducible research: Rigorous quality control and large-scale manufacturing capabilities ensure consistent performance from every lot of iCell Microglia (>80% TREM2+, IBA1+, CX3CR1+).
- Rapid results: iCell Microglia are fully differentiated cells (not precursors) allowing for more experiments in less time.
- Disease lines available: TREM2, SNCA and MeCP2 engineered mutants and isogenic control.
Publications
Fenebrutinib, a Bruton's tyrosine kinase inhibitor, blocks distinct human microglial signaling pathways Langlois J, Lange S, Ebeling M, Macnair W, Schmuck R, Li C, DeGeer J, Sudharshan T, Yong V, Shen Y, Harp C, Collin L, Keaney J J Neuroinflammation. 21(1):276 (2024)
Single-cell transcriptomic and functional studies identify glial state changes and a role for inflammatory RIPK1 signaling in ALS pathogenesis Zelic M, Blazier A, Pontarelli F, LaMorte M, Huang J, Tasdemir-Yilmaz O, Ren Y, Ryan S, Shapiro C, Morel C, Krishnaswami P, Levit M, Sood D, Chen Y, Gans J, Tang X, Hsiao-Nakamoto J, Huang F, Zhang B, Berry JD, Bangari DS, Gaglia G, Ofengeim D, Hammond TR Immunity. 54(4):961-979 (2025)
Microglia integrated neural spheroids enable neuroinflammatory responses and correct network dysfunction induced by alpha-synuclein mutation Ferrer M, Zhang J, Lim Y, Medina A, Chen Y, Carvajal M, Lee E Research Square. (2025)