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iCell Brain Microvascular Endothelial Cells
The blood-brain barrier (BBB) functions to maintain a tightly controlled microenvironment around the brain. The barrier-forming cells of the BBB are brain microvascular endothelial cells (BMECs), which play a crucial role in maintaining homeostasis by regulating the selective exchange of substances between compartments, protecting against pathogens, and preserving tissue integrity.
The inherent power of induced pluripotent stem cell (iPSC) technology provides access to these specialized cell types, enabling reliable manufacturing to generate a consistent supply of cells at scale. FUJIFILM Cellular Dynamics Inc., a market leader in iPSC technology and innovation, is launching a component of its popular human iPSC-derived BBB isogenic kit—the Brain Microvascular Endothelial Cells Kit—in response to popular demand. The iCell Brain Microvascular Endothelial Cells Kit includes iCell BMECs and media that support their long-term survival and superior functional performance, as assessed by transendothelial electrical resistance (TEER) assays.
$1,950.00
Products
Kits
- 3M, Cryopreserved iCell Brain Microvascular Endothelial Cells, 01279
- 200 µl, iCell Plating Supplement A
- 100 ml, iCell BMEC Maintenance Medium
genetic status
quantity (Cells Per Vials)
catalog #
Catalog #: R1236
iCell Brain Microvascular Endothelial Cells
From
$1,950.00
Product Overview
The blood-brain barrier (BBB) functions to maintain a tightly controlled microenvironment around the brain. The barrier-forming cells of the BBB are brain microvascular endothelial cells (BMECs), which play a crucial role in maintaining homeostasis by regulating the selective exchange of substances between compartments, protecting against pathogens, and preserving tissue integrity.
The inherent power of induced pluripotent stem cell (iPSC) technology provides access to these specialized cell types, enabling reliable manufacturing to generate a consistent supply of cells at scale. FUJIFILM Cellular Dynamics Inc., a market leader in iPSC technology and innovation, is launching a component of its popular human iPSC-derived BBB isogenic kit—the Brain Microvascular Endothelial Cells —in response to popular demand. The iCell Brain Microvascular Endothelial Cells is provided with media and supplements that support their long-term survival and superior functional performance, as assessed by transendothelial electrical resistance (TEER) assays.
iCell Brain Microvascular Endothelial Cells and their media have the potential to integrate with high-throughput screening assays, emerging organ-on-a-chip technologies, and other 3D cell culture systems. This capability offers an exciting new avenue for the drug discovery community to advance the understanding of BMEC function in relation to human health and disease.
The iCell Brain Microvascular Endothelial Cells:
- Utilizes our newly developed cryopreserved iCell Brain Microvascular Endothelial Cells, from donor 01279 and also includes our novel plating and BMEC Maintenance medium.
- Achieves superior transendothelial electrical resistance (TEER) values within 5 days.
- Enables high throughput screening studies.
Components:
Catalog #R1236
iCell Brain Microvascular Endothelial Cells, 1 Vial: ≥ 3 x 10^6 cells/vial
- 3M, Cryopreserved iCell Brain Microvascular Endothelial Cells, 01279
- 200 µl, iCell Plating Supplement A
- 100 ml, iCell BMEC Maintenance Medium
Donor Background
Sex:
MaleRace:
CaucasianCell Type:
Brain Microvascular Endothelial CellsSource Line:
01279Modeled Disease Phenotype:
Apparently Healthy NormalDonor Status:
Apparently Healthy NormalTissue Source:
BloodAge At Sampling:
50-59Engineered Line:
NoDisease Status:
Apparently Healthy Normal (AHN)Our regular business hours are 9:00am to 5:00pm Central Time (USA)
Need Help With This Product?
Our specialists are here to help you find the best product for your application.
Our regular business hours are 9:00am to 5:00pm Central Time (USA)
Technical Docs
PROTOCOLS
USER DOCS
User's Guides
iCell Brain Microvascular Endothelial Cell User Guide
Biosafety Document
iCell BMEC, 01279 Biosafety Document
Safety Data Sheet
iCell Plating Supplement A Safety Data Sheet - Asia
Safety Data Sheet
iCell Plating Supplement A Safety Data Sheet - Europe
Safety Data Sheet
iCell Plating Supplement A Safety Data Sheet
Safety Data Sheet
iCell BMEC Maintenance Medium Safety Data Sheet
Safety Data Sheet
iCell BMEC Maintenance Medium Safety Data Sheet - Asia
Performance Data
Characterization of iPSC-derived Brain Microvascular Endothelial Cells
Brain microvascular endothelial cells (BMEC) are unlike other vascular endothelial cells lining peripheral blood vessels in that they display distinctive morphological, structural, and functional features. iCell® BMEC have cobblestone morphology as tightly packed cells with uniform size and clear cell boundaries. iCell BMEC marker expression (by ICC and flow cytometry) reveals characteristic endothelial markers (ZO-1, Claudin 5), transporters (GLUT1, CD98hc), and efflux/ influx proteins (BCRP, P-gp, MRP1, TfRc; these data not shown here). iCell BMEC exhibit much higher TEER functionality compared to iCell Endothelial Cells.
Receptor-Mediated Transcytosis Assay using iCell® Brain Microvascular Endothelial Cells Kit
A primary application of the iCell® Brain Microvascular Endothelial Cells is to measure receptor-mediated transcytosis (RMT). We have characterized expression of the transferrin receptor (TfR) by Western Blot and ICC (on Day 5 of iCell BMEC culture) and investigated timing of uptake/endocytosis of pHrodo red-labeled transferrin on an IncuCyte S3 (Sartorius). Kinetics of the uptake/endocytosis of pHrodo red-labeled transferrin added on day 3 (blue), day 4 (red) and day 5 (green) as compared to control is shown suggesting TfRC is expressed and also functional in iCell BMEC’s.
Minimal Diffusion of Lucifer Yellow Thru BMEC monolayer
A permeability assay using transwells was developed to measure fluorescence intensity of a molecule (e.g., lucifer yellow) permeating through a cultured monolayer of iCell BMEC over time. Assay was performed on Day 5 post-thaw and LY fluorescence signal from the apical to basolateral side was compared. The apparent permeability coefficient (Papp) was calculated to measure integrity of barrier function. Data shows minimal diffusion through the monolayer (green bars) compared to an empty transwell (no cells; gray bars). Papp measurements below 1 x 10-6 cm/sec formation of tight junctions and strong barrier function.
