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iCell Macrophages 2.0 Kit, 01279

Power your research with iCell® Macrophages 2.0, a fully functional human macrophage cell line derived from induced pluripotent stem cells (iPSCs).

  • Phagocytic activity iCell Macrophages 2.0 have phagocytic capabilities equivalent to human monocyte-derived macrophages.
  • Macrophage polarization Like human primary macrophages, these cells adapt their phenotypes in response to distinct stimuli.
  • Biological relevance Incorporating macrophages into in vitro models enhances physiological relevance and translatability.
  • Homeostatic maintenance iCell Macrophages 2.0 act as tissue-resident macrophages when co-cultured with isogenic cells.
  • Fast recovery This terminally differentiated human macrophage cell line is ready to use immediately post-thaw for up to 14 days.
$1,600.00
Catalog # R1186
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Products

Kits

  • 1 vial Cryopreserved iCell Macrophages 2.0, 01279
  • User's Guide
  • Additional reagents required; see the Quick Guide

genetic status

quantity (Cells Per Vials)

catalog #

Catalog #: R1186

iCell Macrophages 2.0 Kit, 01279

From
$1,600.00

Product Overview

Macrophages are phagocytic cells of the innate immune system. These cells are involved in degradation of cellular debris and pathogens, antigen presentation, and cytokine and chemokine release. Macrophages occur in almost all tissues, support maintenance of homeostasis, repair, and remodeling, especially after injury. Macrophages also play key roles within the tumor microenvironment, especially in angiogenesis and matrix remodeling.

iCell Macrophages 2.0 provide a simple, reproducible, and biologically relevant source of macrophages to model and interrogate innate immunity, inflammatory responses, and tissue function and stimulation. iCell Macrophages 2.0 are functionally naïve macrophages able to respond to pro- or anti-inflammatory stimulation, perform phagocytosis, and are ideal for use throughout your process workflow.

Reproducible Results High purity cells from FCDI’s Quality Controlled manufacturing process yields consistent, uniform macrophage function Speed to Assay Ready-to-use at thaw for rapid results; less time than differentiating from Peripheral Blood Monocytes or THP-1 leukemia cells Workflow Compatible Scalable, adaptable, and robust; able to be maintained for up to 14 days in culture

Components:

Catalog #R1186
iCell Macrophages 2.0 Kit, 01279, 1 Vial: ≥ 1 x 10^6 cells/vial
  • 1 vial Cryopreserved iCell Macrophages 2.0, 01279
  • User's Guide
  • Additional reagents required; see the Quick Guide

Donor Background

Sex:
Male
Race:
Caucasian
Cell Type:
Macrophages
Modeled Disease Phenotype:
Apparently Healthy Normal
Donor Status:
Healthy
Tissue Source:
Blood
Age At Sampling:
50-59
Engineered Line:
No
Disease Status:
Apparently Healthy Normal (AHN)

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(877) 320-6688

 

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Our regular business hours are 9:00am to 5:00pm Central Time (USA)

Need Help With This Product?

Our specialists are here to help you find the best product for your application.

Call Us

(877) 320-6688

 

OR

Our regular business hours are 9:00am to 5:00pm Central Time (USA)

Performance Data

iCell Macrophages 2.0 Morphology

iCell Macrophages 2.0 exhibit a classic macrophage morphology, with an adherent monolayer, large rounded cells shape, cytoplasmic vacuoles, and pseudopodia. iCell Macrophages 2.0 also stain positive for the classic hematologic Wright Stain.

Inflammatory and Anti-Inflammatory Cytokine Response

iCell Macrophages 2.0 have a robust cytokine response to pro- and anti- inflammatory stimulation, comparable to primary macrophages. Cells were plated into 96-well plates and allowed to recover for 3 days prior to treatment with the proinflammatory stimuli LPS (1 ug/ml) or IFNγ (50 ng/ml) or the anti-inflammatory stimuli IL-4 (50 ng/ml) and IL-13 (50 ng/ml). After 24 hours, supernatant cytokine levels were assayed using the Luminex Multiplex Assay system.

Phagocytosis of pHrodo Labeled Bioparticles

iCell Macrophages 2.0 were incubated with 100 ug/ml pHrodo labeled opsonized S. aureus bioparticles 3 days post-thaw. Images were captured on the IncuCyte S3 after 15 minutes and 5 hours. Overlay of phase and red fluorescence images.

Functional Antibody Dependent Phagocytosis

iCell Macrophages 2.0 and peripheral blood monocyte-derived macrophages were plated in 50 ng/ml IFNγ.  After 24 hours, rituximab (αCD20) and targets were added simultaneously.  Targets were labeled with CllTrace-FarRed or CellTrace-CFSE.  After 4 hours, cells were collected and stained with αCD11b to determine the % of double positive macrophages.

Product Highlights

iCell Macrophages 2.0 Applications

  • Full Function Macrophages - Biologically relevant source of human macrophages
  • Reproducible Results - High purity cells with consistent, uniform macrophage function
  • Speed to Assay - Ready-to-use at thaw for rapid results
  • Workflow Compatible - Scalable, adaptable, robust cells