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iCell Induced Excitatory Neurons, 01279

Human iPSC-derived excitatory neurons from a Caucasian male (aged 50-59) with a healthy phenotype, differentiated from blood-derived iPSCs.

Fuel efficient and economical research with functional human induced pluripotent stem cell (iPSC)-derived iCell® Induced Excitatory Neurons 01279.

  • Biological relevance iCell Induced Excitatory Neurons express key glutamatergic markers and form functional neural networks.
  • Simple implementation These neurons arrive fully differentiated—simply thaw and plate for consistent, mature excitatory cultures.
  • Economical experiments Optimized for low plating density, these cells decrease cost per experiment while increasing assay throughput.
  • Isogenic options Choose from healthy or diseased iCell Induced Excitatory Neurons and co-culture with other isogenic cell types.
  • Broad utility These neurons support diverse research, including neurodegeneration studies, drug development, and toxicity testing.
Catalog # GIEN01279
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  • Cryopreserved iCell Induced Excitatory Neurons, 01279
  • iCell Neural Supplement B (2 mL)
  • iCell Nervous System Supplement (1 mL)
  • iCell Plating Supplement B (100 µL)
  • User’s Guide

genetic status

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Catalog #:

Induced Excitatory Neurons differentiated from human iPS cells, frozen

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$658.00

Cells Only

  • Cryopreserved iCell Induced Excitatory Neurons, 01279
  • User’s Guide

genetic status

Quantity (Cells Per Vials)

Catalog #

Catalog #:

Induced Excitatory Neurons differentiated from human iPS cells, frozen

From
$558.00

Product Overview

iCell Induced Excitatory Neurons are designed to accelerate and economize your neurodegenerative and neurodevelopmental disease drug discovery. These cells are a highly pure population of human glutamatergic neurons, expressing characteristic receptors (AMPA) and transporters (VGLUT1, VGLUT2). Derived from induced pluripotent stem (iPS) cells engineered to overexpress neurogenin 2 (NGN2), these neurons are fully differentiated at thaw and do not require further exposure to doxycycline or anti-mitotic molecules.

iCell Induced Excitatory Neurons offer a convenient and reliable source of human excitatory neuronal cells for use in life science research, early preclinical discovery, and toxicity testing.


Benefits of iCell Induced Excitatory Neurons:

  • High purity (>90% purity) and consistent across lots
  • Low plating densities for economical preclinical screening experiments
  • Fully differentiated at thaw, with no additional doxycycline required
  • Isogenic donor with iCell Microglia and iCell Astrocytes 2.0
  • Display robust and synchronous MEA activity
  • Disease models available

Disease models are available as iCell Induced Excitatory Neurons: iCell Induced Excitatory Neurons GRN R493X HZ KO (Frontotemporal dementia) Inquire about custom options.

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Need Help With This Product?

Our specialists are here to help you find the best product for your application.

Call Us

(877) 320-6688

 

OR

Our regular business hours are 9:00am to 5:00pm Central Time (USA)

Performance Data

iCell Induced Excitatory Neurons Low Plating Density Extends Value per Vial

iCell Induced Excitatory Neurons can be plated at a low density (0.625x105 cells/cm2) enabling multiple plates to be seeded from one cryovial. This feature facilitates economical high throughput research workflows using a highly pure population of iPSC-derived glutamatergic neurons.

iCell Induced Excitatory Neurons have Consistently High Purity

Multiple lots of iCell Induced Excitatory Neurons were evaluated at thaw for neural purity using flow cytometry. A) Representative flow lots for βIII-Tubulin (Tuj-1; neuron marker) and Nestin (neural progenitor) show >98% Tuj-positive cells and very low presence of Nestin-positive impurities. Isotype antibody control flow plots are shown. B) Tuj-1 purity across lots show >90% purity, with an average purity ~ 96%.

iCell Induced Excitatory Neurons have a Consistent Glutamatergic Gene Expression Profile

Multiple lots of iCell Induced Excitatory Neurons were evaluated at thaw for gene expression. Data reveal a consistent and primarily excitatory glutamatergic neuronal population, with high expression of vesicular glutamate transporters (vGlut1 and vGlut2), and AMPA receptor subunits. Consistent expression of synaptic genes (SYN1 and PSD95), neuronal markers (TUBB33, MAP2) is also observed. This gene expression profile is consistent with published results on NGN2-induced neurons.

iCell Induced Excitatory Neurons Consistently Develop Neural Network Activity on MEA

iCell Induced Excitatory Neurons were co-cultured with iCell Astrocytes 2.0 on Cytoview plates for 3 weeks prior to MEA recording on the Axion Maestro. Representative plots across 3 different lots of iCell Induced Excitatory Neurons shows their consistent for neural network formation.

Timecourse of iCell Induced Excitatory Neurons Network Development

iCell Induced Excitatory Neurons and iCell Astrocytes 2.0 were cultured on a 24-well HD-MEA plate and activity measured every 7 days for 6 weeks using Maxwell Biosystems MaxTwo Multi-Well HD-MEDA System. A) Representative raster plot and network activity histogram at 28 days post thaw. B) iCell Induced Excitatory Neurons network activity develops over time as visualized by quantifying Burst Frequency, Spikes within Bursts, Burst Duration, and Inter Burst Interval.

iCell Induced Excitatory Neurons Exhibit Calcium Oscillations in NeuroSpheres

iCell Induced Excitatory Neurons were cultured with iCell Astrocytes 2.0 following the iCell NeuroSphere Application Protocol. Each well contained 25,000 cells at varying Astrocyte to Neuron ratios (1:1, 1:2, and 1:4). A) Representative images of an iCell NeuroSphere after 3 weeks. B) After 24 days in culture, neurospheres were loaded with a calcium indicator and calcium activity monitored. iCell Induced Excitatory Neuron neurospheres displayed calcium oscillations. Oscillation patterns varied depending on the ratio of neurons to astrocytes.

Product Highlights

iCell Induced Excitatory Neurons Highlights

  • Economical – Low plating density enables 1 vial to fill three 96-well plates.
  • Highly Glutamatergic – Overexpression of neurogenin-2 produces a highly pure excitatory neuron population.
  • Ready-to-use – Cells are fully differentiated at thaw, with no additional doxycycline required.
  • Rapid maturation – Immediate neurite outgrowth and development of neural network activity in 2-3 weeks when co-cultured with iCell Astrocytes 2.0.
  • Isogenic co-culture – Derived from the same donor iPSC as our iCell Microglia and iCell Astrocytes 2.0, enabling fully isogenic neural co-culture.