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iCell Astrocytes 2.0, 01279
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Kits
- Cryopreserved iCell Astrocytes 2.0, 01279
- iCell Astrocytes 2.0 Medium (50mL)
genetic status
Quantity (Cells Per Vials)
Catalog #
Catalog #:
Astrocytes 2.0 differentiated from human iPS cells, frozen
From
$1,245.00
Cells Only
- Cryopreserved iCell Astrocytes 2.0, 01279
genetic status
Quantity (Cells Per Vials)
Catalog #
Catalog #:
Astrocytes 2.0 differentiated from human iPS cells, frozen
From
$1,055.00
Product Overview
iCell Astrocytes 2.0 are serum-free and ready for isogenic co-culture. These iPSC-derived human astrocytes are a highly pure population of human astrocytes derived from induced pluripotent stem (iPS) cells using FCDI’s proprietary differentiation protocol. They have an extended purity and functional quality control profile and are cultured in serum-free medium to provide a robust, reliable, and consistent off-the-shelf option for human astrocytes.
iCell Astrocytes 2.0 express known astroglia markers (CD44, CD49f, S100β, GFAP) and display functional properties of astrocytes, including glutamate uptake, IL-6 secretion in response to inflammatory stimuli, and enhancement of neural network formation in co-culture with iCell® GlutaNeurons. These cells provide a reliable source of human astrocytes suitable for use in targeted drug discovery, toxicity testing, and other discovery research.
iCell Astrocytes 2.0 benefits:
- Compatible for isogenic co-culture (iPSC donor 01279)
- Defined serum-free post-thaw culture minimizes baseline inflammatory cytokine secretion.
- Robust proinflammatory response to IL-6 stimulation enables neuroinflammations studies.
- Consistent glutamate uptake across lots demonstrates performance reproducibility.
- Improves neural activity maturation on MEA platforms.
Components:
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Need Help With This Product?
Our specialists are here to help you find the best product for your application.
Our regular business hours are 9:00am to 5:00pm Central Time (USA)
Technical Docs
PROTOCOLS
Application Protocols
Tri-Culture of iCell Microglia with iCell Neuronal Cell Types and iCell Astrocytes
USER DOCS
User's Guides
iCell Astrocytes 2.0 User's Guide
Datasheet
iCell Astrocytes 2.0 Data Sheet
Biosafety Document
iCell Astrocytes 2.0 Biosafety Document
Safety Data Sheet
iCell Astrocytes 2.0 Medium Safety Data Sheet
Safety Data Sheet
iCell Cell-Based Products Safety Data Sheet - Asia
Safety Data Sheet
iCell Cell-Based Products Safety Data Sheet
Performance Data
iCell Astrocytes 2.0 are Highly Pure Population of Human iPSC-derived Astrocytes
Top) iCell Astrocytes 2.0 were analyzed at thaw using flow cytometry, showing a highly pure population of astrocytes as identified by expression of CD44, GFAP, and CD49f. Bottom) Representative immunocytochemistry images of iCell Astrocytes 2.0 at 7 days post thaw showing expression of CD44, CD49f, S100b, and GFAP.
Consistent Glutamate Uptake Across Lots of iCell Astrocytes 2.0
iCell Astrocytes 2.0 from four differentiation lots (Lots 1-4) were plated at 5x104 cells/cm2 and cultured for 7 days using iCell Astrocyte Medium. iCell Astrocytes 2.0 were cultured for 1 hour with Glutamate (20 µM) and evaluated for glutamate uptake. Data show consistent glutamate uptake across lots. Additon of 300μM DL-threo-β-Benzyloxyaspartic acid (TBOA), a selective inhibitor of glutamate excitatory amino-acid transporters, reducd glutamate uptake in all lots.
Neuroinflammatory Response in iCell Astrocytes 2.0
iCell Astrocytes 2.0 cultured for 7 days using iCell Astrocytes 2.0 Medium. Astrocytes were the stimulated for 24 hours with IL-1β at multiple dose concentrations. Cell culture supernatants were collected and analyzed for IL-6 secretion using ELISA. iCell Astrocytes 2.0 display low basal IL-6 secretion which dose-dependently increased with IL-1β addition. Comparatively, iCell Astrocytes, 01434 (Cat # C1037) show higher basal IL-6 and reduced secretion following stimulation.
iCell Astrocytes 2.0 promote Network Formation in Co-culture with iCell GlutaNeurons
iCell Astrocytes 2.0 were plated with iCell GlutaNeurons in a 1:6 ratio and cultured following the FCDI application protocol to co-culture for multielectrode array (MEA) recording on the Axion Maestro. Representative MEA raster plots at 21 days in vitro indicate improved neural network synchrony of GlutaNeurons in co-culture with iCell Astrocytes 2.0. Two lots of iCell Astrocytes 2.0 were tested and display similar network maturation compared to iCell GlutaNeurons in culture alone.
iCell Astrocytes Modulate Calcium Oscillations in NeuroSpheres
iCell Astrocytes 2.0 were cultured with iCell Induced Excitatory Neurons following the iCell NeuroSphere Application Protocol. Each well contained 25,000 cells at varying Astrocyte to Neuron ratios (1:1, 1:2, and 1:4). (A) Representative images of an iCell NeuroSphere after 3 weeks. (B) After 24 days in culture, neurospheres were loaded with a calcium indicator and calcium oscillations were recorded. Representative oscillation plots demonstrate the effect of different astrocyte to neuron ratio affects calcium oscillation activity.
Product Highlights
iCell Astrocytes 2.0 Highlights
- Defined culture conditions – iCell Astrocytes 2.0 are shipped with an optimized serum-free medium. Simply thaw and use.
- High purity – iCell Astrocytes 2.0 are >90% CD44+, with expression of key markers like CD49f, GFAP, S100β, GLAST, and Aquaporin-4.
- Robust function – iCell Astrocytes 2.0 show consistent glutamate uptake and improved neural network maturation on multielectrode array (MEA) platforms.
- Low activation – Serum-free culture conditions reduce iCell Astrocytes 2.0 basal cytokine secretion enabling neuroinflammations studies.