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iCell GFP Microglia, 01279
Human iPSC-derived GFP microglia from a Caucasian male (age 50-59) with a healthy phenotype, differentiated from blood-derived iPSCs.
Products
Kits
- 1 vial Cryopreserved iCell GFP Microglia, 01279
- 50ml iCell Glial Base Medium
- 0.5ml iCell Microglia Supplement A (100x)
- 0.5ml iCell Microglia Supplement B (100x)
- 1ml iCell Neural Supplement C (50X)
- iCell Microglia Quick Guide
- Additional reagents required; see Quick Guide
genetic status
Quantity (Cells Per Vials)
Catalog #
Catalog #:
Microglia differentiated from human iPS cells, frozen
From
$2,395.00
Cells Only
- 1 vial Cryopreserved iCell GFP Microglia, 01279
- iCell Microglia Quick Guide
- Additional reagents required; see Quick Guide
genetic status
Quantity (Cells Per Vials)
Catalog #
Catalog #:
Microglia differentiated from human iPS cells, frozen
From
$2,095.00
Product Overview
Microglia, the resident immune cells in the central nervous system, respond to infection, insult, or neurodegeneration by releasing cytokines, increasing phagocytic capacity, and undergoing morphological changes between an ameboid and ramified structure. iPSC-derived microglia are an instrumental tool in neurodegenerative and neuroinflammatory disease research, providing unlimited access to human microglia and enabling interrogation of cell activation pathways and function. These cells are increasingly being used in 2D and 3D co-culture, where evaluating microglia motility and morphology can be challenging.
To enable visualization of microglia in complex cultures, we engineered and manufactured iCell Microglia that express cytosolic. iCell GFP Microglia allow for real-time imaging in complex co-culture, helping researchers evaluate microglia viability, morphology, and motility.
Components:
Our regular business hours are 9:00am to 5:00pm Central Time (USA)
Need Help With This Product?
Our specialists are here to help you find the best product for your application.
Our regular business hours are 9:00am to 5:00pm Central Time (USA)
Technical Docs
PROTOCOLS
Application Protocols
Immunofluorescent Labeling of iCell Microglia
Application Protocols
Labeling Amyloid Beta with pHrodo Red
Application Protocols
Measuring Microglia Phagocytosis: Kinetic Imaging on the IncuCyte
Application Protocols
Tri-Culture of iCell Microglia with iCell Neuronal Cell Types and iCell Astrocytes
USER DOCS
Quick Guide
iCell Microglia Quick Guide
Datasheet
iCell Microglia
Biosafety Document
iCell Microglia Biosafety Document
Safety Data Sheet
iCell Neural Supplement C (50X) Safety Data Sheet - Asia
Safety Data Sheet
iCell Neural Supplement C (50X) Safety Data Sheet
Safety Data Sheet
iCell Glial Basal Medium Safety Data Sheet - Asia
Safety Data Sheet
iCell Glial Basal Medium Safety Data Sheet
Safety Data Sheet
iCell Neural Supplement C (50X) Safety Data Sheet
Safety Data Sheet
iCell Neural Supplement C (50X) Safety Data Sheet - Asia
Safety Data Sheet
iCell Cell-Based Products Safety Data Sheet - Asia
Safety Data Sheet
iCell Cell-Based Products Safety Data Sheet
Safety Data Sheet
iCell Glial Base Medium Safety Data Sheet
Safety Data Sheet
iCell Glial Base Medium Safety Data Sheet - Asia
Safety Data Sheet
iCell Neural Supplement C Safety Data Sheet
Safety Data Sheet
iCell Neural Supplement C Safety Data Sheet - Asia
Safety Data Sheet
iCell Microglia Supplement A Safety Data Sheet
Safety Data Sheet
iCell Microglia Supplement A Safety Data Sheet - Asia
Safety Data Sheet
iCell Microglia Supplement B Safety Data Sheet
Safety Data Sheet
iCell Microglia Supplement B Safety Data Sheet - Asia
Performance Data
Robust GFP Expression
iCell GFP Microglia were cultured for 7 days post-thaw and then stained using the Iba-1 antibody (Cat # 0198-19741). Overlay shows robust GFP expression with the majority of microglia co-expressing GFP and Iba-1.
Measure Neuroinflammatory Responses in Tri-Culture
iCell GFP Microglia were cultured with iCell Astrocytes 2.0 (Cat # C1249) and iCell GABANeurons (Cat # C1008) using the iCell Tri-Culture Application Protocol. After 13 days of co-culture, cells were stimulated with LPS (48 hours) prior to fixation and staining. iCell GFP Microglia co-localize with Iba-1 (Cat # 0198-19741) staining undergo qualitative changes in morphology following LPS stimulation.
Visualize Microglia in 3D iCell NeuroSpheres
Immune competent 3D brain cultures are increasingly desired for New Approach Methodologies (NAM) enabling neurodegenerative drug screening. Using iCell GFP Microglia we demonstrate that microglia can be incorporated into 3D iCell NeuroSpheres. (Left) Immunocytochemistry showing GFP Microglia integrated into iCell NeuroSpheres (iCell GlutaNeurons and iCell Astrocytes 2.0). (Right) Similarly, iCell GFP Microglia incorporate into neurospheres composed of various iCell neuronal cell products, demonstrating the robustness and flexibility of incorporation into different models.
Publications
Fenebrutinib, a Bruton's tyrosine kinase inhibitor, blocks distinct human microglial signaling pathways Langlois J, Lange S, Ebeling M, Macnair W, Schmuck R, Li C, DeGeer J, Sudharshan T, Yong V, Shen Y, Harp C, Collin L, Keaney J J Neuroinflammation. 21(1):276 (2024)
Single-cell transcriptomic and functional studies identify glial state changes and a role for inflammatory RIPK1 signaling in ALS pathogenesis Zelic M, Blazier A, Pontarelli F, LaMorte M, Huang J, Tasdemir-Yilmaz O, Ren Y, Ryan S, Shapiro C, Morel C, Krishnaswami P, Levit M, Sood D, Chen Y, Gans J, Tang X, Hsiao-Nakamoto J, Huang F, Zhang B, Berry JD, Bangari DS, Gaglia G, Ofengeim D, Hammond TR Immunity. 54(4):961-979 (2025)
Microglia integrated neural spheroids enable neuroinflammatory responses and correct network dysfunction induced by alpha-synuclein mutation Ferrer M, Zhang J, Lim Y, Medina A, Chen Y, Carvajal M, Lee E Research Square. (2025)