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iCell Hepatic Stellate Cells, 01279
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- iCell Hepatic Stellate Cells, 01279
- HSC 3D Monoculture Quick Guide
- HSC 2D Monoculture Quick Guide
- Hep/HSC 3D Co-culture Quick Guide
genetic status
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Catalog #:
Hepatic Stellate Cells differentiated from human iPS cells, frozen
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$1,500.00
Cells Only
- iCell Hepatic Stellate Cells, 01279
genetic status
Quantity (Cells Per Vials)
Catalog #
Catalog #:
Hepatic Stellate Cells differentiated from human iPS cells, frozen
From
$1,500.00
Product Overview
Hepatic stellate cells are liver specific mesenchymal cells found in the perisinusoidal space of Disse. These cells are the major storage site for Vitamin A, holding up to 80% of the retinyl palmitate found in the body. HSCs become activated in response to acute and chronic liver injury, transitioning into a myofibroblast state, depositing extracellular matrix and growth factors.
iCell Hepatic Stellate Cells provide a simple, reproducible, and biologically relevant source of quiescent HSC to model and interrogate liver physiology in monoculture and co culture conditions. iCell Hepatic Stellate Cells are functionally quiescent, able to store Vitamin A and respond to stimulation. These cells are ideal for use throughout your process workflow.
- Reproducible Results High purity cells for consistent, uniform function; unlimited cells from a single donor
- Speed to Assay Ready-to-use at thaw for rapid results
- Workflow Compatible Scalable, adaptable, and robust
- Co-culture Ready Compatible with hepatocyte media; available with isogenic hepatocytes and macrophages
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Our specialists are here to help you find the best product for your application.
Our regular business hours are 9:00am to 5:00pm Central Time (USA)
Technical Docs
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Datasheet
iCell Hepatic Stellate Cells Data Sheet
Performance Data
Vitamin A Storage
Out of thaw, iCell Hepatic Stellate Cells are in a quiescent state. One of the hallmarks of this functional state in HSCs is the ability to store Vitamin A, leading the cells to become auto fluorescent in response to excitation at the 405nm wavelength typically used for DAPI. This phenomenon is readily observable when iCell Hepatic Stellate Cells are cultured in the presence of 10μM Vitamin A.
Collagen Secretion after Activation
iCell Hepatic Stellate Cells can be maintained in the quiescent state in 3D monoculture, exhibiting low to undetectable levels of secreted pro-collagen Iα. iCell HSC can be activated by plating to 2D culture or treated with TGFβ in 3D culture. Upon activation, iCell Hepatic Stellate Cells significantly upregulate pro-collagen Iα secretion for an easy to assay readout of HSC functional state.
Hepatocyte Co-Culture Ready
iCell Hepatic Stellate Cells are easy to integrate into hepatocyte workflows. iCell Hepatocytes 2.0 were harvested to make 3D spheroids. At aggregation, isogenic iCell HSC were included from thaw at 15% of the total cell population alongside hepatocyte only spheroids with 0% HSC. Both conditions were maintained in the recommended hepatocyte spheroid maintenance media. After 1 week in culture, spheroids were stained and imaged for the HSC quiescent marker LRAT. iCell Hepatic Stellate Cells can be found running throughout the co-culture spheroids.
