iCell DopaNeurons, 01279

iCell DopaNeurons Develop Synchronous Network Activity over Time iCell DopaNeurons cultured in complete BrainPhys medium exhibit high levels of neuronal activity as soon as day 2 post-plating and routinely incorporate ≥12 of 16 active electrodes/well of the MEA plate. Importantly, these cells display network-wide bursts in each well with optimal synchronous neuronal activity observed from day 18 post-plating. (A) As shown in the raster plots, where the white lines indicate spikes or neuronal action potentials, Poisson channel bursting is observed during the first week post-plating (day 6) as indicated by the red tick marks. This bursting rate (bursts per minute, bpm) increases over time (day 14), and eventually the neuronal activity develops into a synchronized network that organizes the entire culture (day 20). The purple boxes around the organized spikes in each raster plot denote inter-spike interval (ISI) network bursts captured by the AxIS Software. The network bursting percentage is defined as the number of spikes that fall within the purple box divided by the total number of tick marks measured during the recording. The higher this percentage is, the more synchronous the neuronal culture is considered to be. (B) The graphs represent mean and SEM values calculated on days 6, 14, and 20 postplating.