No, iCell
® Cardiomyocytes Plating and Maintenance Medium used with iCell Cardiomyocytes and iCell Cardiomyocytes
2 are antibiotic- and antimitotic-free. Yes, you can add antibiotics to your culture as a preventative measure against inadvertent bacterial contamination. For certain applications (MEA, Seahorse, others) we have added 25 µg/ml gentamicin
(ThermoFisher Scientific, Cat. No. 15750-060) as early as day 2 post-thaw to iCell Cardiomyocytes when switching from Plating Medium to Maintenance Medium. Alternatively, iCell Cardiomyocytes will tolerate 1X penicillin-streptomycin (
ThermoFisher Scientific, Cat. No. 15140122). For iCell Cardiomyocytes
2, you can add an antibiotic when switching to Maintenance Medium 4 hours post thaw. While we have not performed thorough assessments of functional impacts of these antibiotics on the cells, we have qualitatively observed no significant impact.