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Do cardiomyocyte media contain antibiotics or antimitotics? Can I add antibiotics to these media and will the addition affect my assays?

No, iCell® Cardiomyocytes Plating and Maintenance Medium used with iCell Cardiomyocytes and iCell Cardiomyocytes2 are antibiotic- and antimitotic-free. Yes, you can add antibiotics to your culture as a preventative measure against inadvertent bacterial contamination. For certain applications (MEA, Seahorse, others) we have added 25 µg/ml gentamicin (ThermoFisher Scientific, Cat. No. 15750-060) as early as day 2....

Can I use iCell Cardiomyocytes to start cultures for hypoxia models?

Yes. iCell® Cardiomyocytes recapitulate the biochemical, electrophysiological, mechanical, and pathophysiological properties of native human cardiac myocytes, you can use these for a variety of applications including hypoxia. Our Application Protocol describes how to induce and quantify hypoxia in iCell Cardiomyocytes as a human-based in vitro model of cardiac ischemia. For more information about hypertrophy models,....

What are some characteristics and features of iCell Cardiomyocytes2, and how do they differ from iCell Cardiomyocytes?

iCell® Cardiomyocytes2, our second version of cardiomyocyte cells, were developed specifically for use on MEA and xCELLigence platforms and derived from the same iPSC clone, making it genetically identical to the first iCell Cardiomyocytes version. We have shown that there are no significant differences between the first version assayed at 14 days post-thaw and iCell....

Are there any special recommendations for using iCell Cardiomyocytes2 in patch clamp applications?

Our Application Protocol demonstrates the utility of and consistency in recording action potentials from iCell® Cardiomyocytes using a manual perforated patch clamp method. The protocol provides instructions for culturing the cardiomyocytes, preparing the perforated patch clamp, preparing saline solutions, identifying suitable cardiomyocytes for patch clamp, filling the recording pipette, forming the gigaohm (GΩ) seal, recording....

What serum-free media do you recommend for iCell Cardiomyocytes2 in MEA and xCELLigence applications? Can I use these media for other assays?

While our iCell® Cardiomyocytes Plating and Maintenance media for iCell Cardiomyocytes2 contain serum and will expose cells to it during the culture conditions recommended in our User Guide, we have performed a complete medium exchange to serum-free conditions before the assay for incubation lasting overnight (exchange the night before assay) or 4 hours (exchange on....

What are the catalog (part) numbers, contents, and product specifications of each iCell Cardiomyocytes2 Kit?

Each iCell® Cardiomyocytes2 Kit contains enough viable cells* for the 96-well plate capacities in the table below and sufficient volumes of iCell Cardiomyocytes Plating Medium and iCell Cardiomyocytes Maintenance Medium necessary for thawing and maintaining the cells in culture for at least 2 weeks. For longer cultures, you can order more Maintenance Medium.** Catalog Number....

What feeding schedule do you recommended iCell DopaNeurons?

We recommend feeding iCell® DopaNeurons on day 2 post-thaw and every 2 to 3 days thereafter. All medium changes should be a 75 - 100% exchange to fresh Maintenance Medium from an aliquot warmed to room temperature. Be sure to use careful aspiration practices that don’t touch the plated cells. To minimize degradation of medium....

What do you recommend for patch clamping iCell DopaNeurons?

We do not have a fully optimized application protocol for culturing iCell® DopaNeurons intended for manual patch clamp and the various patch clamp rigs. Instead, we offer the following starting point recommendations to adjust as needed for your specific setup. General Protocol Recommendations: Assay ECM: Precoated PDL/laminin 12-mm glass coverslips; uncoated 15-mm plastic or glass....

What do you recommend for using iCell DopaNeurons on Axion Maestro MEAs?

We have an Application Protocol for culturing iCell® DopaNeurons on the 48-well multielectrode arrays (MEAs) from Axion BioSystems and subsequent recording on their Maestro MEA System. This Application Protocol describes how to thaw, plate, and culture iCell DopaNeurons on the Maestro MEA System and provides basic instructions for data acquisition and analysis. General Protocol Recommendations:....

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