TMEM175 is an emerging and provocative therapeutic target for Parkinson’s Disease (PD) treatment. Mutations in TMEM175 are found across sporadic PD patients and are relatively common in the general population. Single nucleotide polymorphisms within TMEM175, specifically 393M/T and 65Q/P, result in altered lysosomal pH regulation and are associated with increased and decreased risk of PD, respectively.
FUJIFILM Cellular Dynamics Inc (FCDI) is excited to offer early access to our new iCell® DopaNeurons TMEM175 mutant lines, featuring the PD risk-associated mutations, 393M/T and 65Q/P along with isogenic controls.
Interested in Early Access? Fill in the form below for additional information on iCell® DopaNeurons TMEM175 Mutants and receive launch notification.
TMEM175 393M/T Risk-Associated Mutation Pairing:
| Product Name | Description | 393 Genotype | 65 Genotype | Donor | |
| Cat # C1028 | iCell DopaNeurons | PD Risk-associated | 393M/T | 65Q/Q | 01279 |
| New! | iCell DopaNeurons TMEM175 393M/M | Mutation-corrected control | 393M/M | 65Q/Q | 01279 |
TMEM175 65Q/P Protective Mutation Pairing:
| Product Name | Description | 393 Genotype | 65 Genotype | Donor | |
| New! | iCell DopaNeurons TMEM175 393M/M | Mutation-corrected control | 393M/M | 65Q/Q | 01279 |
| New! | iCell DopaNeurons TMEM175 393M/M, 65Q/P | Protective | 393M/M | 65Q/P | 01279 |
Figure 1. iCell DopaNeuron TMEM175 mutants form active neural networks (synchronized bursting) when cultured and recorded on microelectrode array (MEA) plates, demonstrating that these cells are highly functional iPSC-derived dopaminergic neurons. TMEM175 DopaNeurons are manufactured to the same purity and viability specifications as our industry-standard iCell DopaNeurons.
Figure 2. (Top) Lysosomal Patch Clamp of iCell DopaNeurons TMEM175 lines was performed at 14 days and current-voltage relationship examined under unstimulated and DCPIB, a TMEM175 agonist, exposure. (Middle) Analysis of TMEM175 current density reveals elevated baseline conductance for the 65Q/P line and equal or reduced conductance for 393M/T and 393T/T lines, respectively, compared to 393M/M. These data agree with previously published data. (Bottom) The mutation did not affect responsiveness to DCPIB as shown by similar EC50 curves between the mutants.
Experiments conducted by Axxam S.p.A.
