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iCell GABANeurons, 01434

Human iPSC-derived cortical neurons from a Caucasian female (aged < 18) with a healthy phenotype, differentiated from fibroblast-derived iPSCs.

Power diverse research with iCell® GABANeurons 01434, relevant and robust human cortical neurons derived from induced pluripotent stem cells (iPSCs).

  • Consistent quality iCell GABANeurons offer >95% pure neuronal populations, primarily consisting of GABAergic neurons.
  • Biological relevance iCell GABANeurons rapidly form functional synapses and exhibit native electrical and biochemical activity.
  • Versatile applications From drug development to neurodegeneration and neurotoxicity, these cells support broad research goals.
  • Long-term stability These cells remain viable and pure in culture for weeks, enabling assessment of acute and sub-chronic responses.
  • Easy implementation Terminally differentiated from iPSCs, iCell GABANeurons are consistent and scalable—simply thaw and plate.
Catalog # GGBN01434
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Products

Kits

  • Cryopreserved iCell GABANeurons, 01434
  • 100 ml Neural Base Medium 1
  • 2 ml Neural Supplement A
  • User’s Guide

genetic status

Quantity (Cells Per Vials)

Catalog #

Catalog #:

GABANeurons differentiated from human iPS cells, frozen

From
$1,795.00

Cells Only

  • Cryopreserved iCell GABANeurons, 01434
  • User’s Guide

genetic status

Quantity (Cells Per Vials)

Catalog #

Catalog #:

GABANeurons differentiated from human iPS cells, frozen

From
$1,695.00

Product Overview

Traditional neuroscience research tools consist largely of rodent primary cell cultures and animal models, which are labor-intensive, costly, and poorly reflective of native human biology. iCell® GABANeurons represent a relevant human in vitro system for modeling and interrogating complex neurological processes and diseases.

iCell GABANeurons are iPS cell-derived mixed populations of human cerebral cortical neurons that exhibit native electrical and biochemical activity. They overcome limitations of existing models by providing the following:

  • >95% pure neuronal population
  • Cell population consists of primarily GABAergic (inhibitory) neurons
  • Rapid formation of neural networks and functional synapses
  • Expression of relevant neurological therapeutic targets and pathways
  • Long-term viability and demonstrated reproducibility
  • Reliable supply in cryopreserved format

More Data, More Publications, More Solutions

There is a broad range of peer-reviewed publications and CDI application protocols and applications notes  to support your research goals including:

  • Alzheimer’s disease research and drug discovery
    Functional tau and β-amyloid toxicity pathways, long-term viability, and flexible product format enable high-throughput screening for novel drug candidates
  • Autism disease research
    Demonstrated prefrontal cortex ionotropic glutamate receptors, GABAA receptor, and synaptic functionality drive interrogation of neurological diseases, such as autism
  • Infectious disease studies
    Susceptibility to varicella zoster virus infection and ability to maintain viral latency and reactivation
  • Therapeutic potency testing
    High sensitivity, dose-response, and biological responses to Clostridium botulinum neurotoxin (BoNT) aid replacement of the gold-standard mouse bioassay for BoNT potency testing
  • Neurotoxicity assessment
    Compatibility with a wide range of high-content, high-throughput platforms enables assessment of pharmaceutical and environmental neurotoxicity

Components:

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Our regular business hours are 9:00am to 5:00pm Central Time (USA)

Need Help With This Product?

Our specialists are here to help you find the best product for your application.

Call Us

(877) 320-6688

 

OR

Our regular business hours are 9:00am to 5:00pm Central Time (USA)

Performance Data

Relevant Neuronal Markers and High Purity

iCell GABANeurons represent a highly pure population comprised primarily of GABAergic neurons with low levels of nestin (neuronal progenitor marker) as demonstrated by immunocytochemistry. These images show iCell GABANeurons, 01434: (A) ß-III tubulin (neuronal marker, green) and nestin (red), 7 days post-plating; (B) punctate staining pattern for the vesicular glutamate transporter 2 (vGLUT2, red) and vesicular GABA transporter (vGAT, green), 14 days post-plating, indicative of glutamatergic and GABAergic neuronal subtypes, respectively; and (C) gamma-aminobutyric acid (GABA, red) and microtubule associated protein 2 (MAP2, green), 14 days post-plating.

Figure 1: iCell GABANeurons Represent a Highly Pure Population of Human Neurons

Figure 2: iCell GABANeurons Exhibit Typical Neuronal Morphology

Expected  GABAergic Neuron Morphology

These images show iCell GABANeurons, 01434 at days 1, 5, and 19 post-plating. The reanimated GABAergic neurons develop branched networks within 24 hours and remain viable and adherent for an extended period in culture (≥14 days).

Neurite Outgrowth Assay

Panels A and B show iCell GABANeurons, 01434 cultured in a 96-well cell culture plate in absence or presence of 50 ng/ml BDNF, respectively. The neurons were stained with calcein AM (green) and Hoechst 33342 (blue). Panels C and D show the segmentation masks generated from calcein AM and Hoechst 33342 fluorescent signals of panels A and B, respectively. Images were acquired using the ImageXpress Micro System, and segmentation masks were generated using the MetaXpress Software.

Figure 3: Representative Effect of BDNF on Neurite Outgrowth of iCell GABANeurons

Figure 4: Quantitative Effect of BDNF on Neurite Outgrowth of iCell GABANeurons

Quantitative Effect of BDNF on Neurite Outgrowth of iCell GABANeurons

Culturing iCell GABANeurons, 01434 in the presence of BDNF for 14 days produced the expected effect of enhancing neurite outgrowth. Data was acquired using the ImageXpress Micro System and quantified using the MetaXpress Software. Data are represented as mean values per number of neuronal cells (mean ± SEM, n = 6 wells).

Product Highlights

More Data, More Publications, More Solutions

There is a broad range of peer-reviewed publications and CDI application protocols and applications notes to support your research goals including:

  • Alzheimer’s disease research and drug discovery
    Functional tau and β-amyloid toxicity pathways, long-term viability, and flexible product format enable high-throughput screening for novel drug candidates
  • Autism disease research
    Demonstrated prefrontal cortex ionotropic glutamate receptors, GABAA receptor, and synaptic functionality drive interrogation of neurological diseases, such as autism
  • Infectious disease studies
    Susceptibility to varicella zoster virus infection and ability to maintain viral latency and reactivation
  • Therapeutic potency testing
    High sensitivity, dose-response, biological responses to Clostridium botulinum neurotoxin
  • Neurotoxicity assessment
    Compatibility with a wide range of high-content, high-throughput platforms enables assessment of pharmaceutical and environmental neurotoxicity

Publications

Three-dimensional Brain-on-chip Model Using Human iPSC-derived GABAergic Neurons and Astrocytes: Butyrylcholinesterase Post-treatment for Acute Malathion Exposure Liu L, Koo Y, Russell T, Gay E, Li Y, Yun Y PLoS One. (2020) 15(3): e0230335. (2020)

Engineered Botulinum Neurotoxin B with Improved Binding to Human Receptors Has Enhanced Efficacy in Preclinical Models Elliott M, Favre-Guilmard C, Liu SM, Maignel J, Masuyer G, Beard M, Boone C, Carré D, Kalinichev M, Lezmi S, Mir I, Nicoleau C, Palan S, Perier C, Raban E, Zhang S, Dong M, Stenmark P, Krupp J (2019) Sci Adv 16;5(1) (2019)

An Epilepsy-associated KCNT1 Mutation Enhances Excitability of Human iPSC-derived Neurons by Increasing Slack KNa Quraishi IH, Stern S, Mangan KP, Zhang Y, Ali SR, Mercier MR, Marchetto MC, McLachlan MJ, Jones EM, Gage FH, Kaczmarek LK (2019) Journal of Neurosci.,1628-18 (2019)

Thiamine Deficiency Induces Endoplasmic Reticulum Stress And Oxidative Stress In Human Neurons Derived From Induced Pluripotent Stem Cells. Wang X, Xu M, Frank JA, Ke ZJ, Luo J. (2017) Toxicol Appl Pharmacol. 320:26-31. (2017)

Comparison Of The Acute Inhibitory Effects Of Tetrodotoxin (TTX) In Rat And Human Neuronal Networks For Risk Assessment Purposes. Kasteel EE, Westerink RH. (2017) Toxicol Lett. 15;270:12-16. (2017)

Protease-activated Receptor-1 Activation by Granzyme B Causes Neurotoxicity that is Augmented by Interleukin-1β Lee PR, Johnson TP, Gnanapavan S, Giovannoni G, Wang T, Steiner JP, Medynets M, Vaal MJ, Gartner V, Nath A (2017) J Neuroinflammation.14(1):131. (2017)

An Indicator Cell Assay for Blood-based Diagnostics Danziger SA, Miller LR, Singh K, Whitney GA, Peskind ER, Li G, Lipshutz RJ, Aitchison JD, Smith JJ (2017) PLoS One.12(6):e0178608. (2017)

Characterization Of GABAA Receptor Ligands With Automated Patch-Clamp Using Human Neurons Derived From Pluripotent Stem Cells. Yuan NY, Poe MM, Witzigmann C, Cook JM, Stafford D Arnold LA. (2016) J Pharmacol Toxicol Methods. 82:109-114. (2016)

Electrical Responses and Spontaneous Activity of Human iPS-Derived Neuronal Networks Characterized for 3-month Culture with 4096-Electrode Arrays Amin H, Maccione A, Marinaro F, Zordan S, Nieus T, Berdondini L (2016) Front Neurosci. 2016 Mar 30;10:121. (2016)

Internalized Tau Oligomers Cause Neurodegeneration by Inducing Accumulation of Pathogenic Tau in Human Neurons Derived from Induced Pluripotent Stem Cells Usenovic M, Niroomand S, Drolet RE, Yao L, Gaspar RC, Hatcher NG, Schachter J, Renger JJ, Parmentier-Batteur S (2015) J Neurosci. 35(42):14234-50. (2015)

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