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iCell GABANeurons, 01279
Human iPSC-derived cortical neurons from a Caucasian male (aged 50-59) with a healthy phenotype, differentiated from blood-derived iPSCs.
Product Overview
Traditional neuroscience research tools consist largely of rodent primary cell cultures and animal models, which are labor-intensive, costly, and poorly reflective of native human biology. iCell® GABANeurons represent a relevant human in vitro system for modeling and interrogating complex neurological processes and diseases.
iCell GABANeurons are iPS cell-derived mixed populations of human cerebral cortical neurons that exhibit native electrical and biochemical activity. They overcome limitations of existing models by providing the following:
- >95% pure neuronal population
- Cell population consists of primarily GABAergic (inhibitory) neurons
- Rapid formation of neural networks and functional synapses
- Expression of relevant neurological therapeutic targets and pathways
- Long-term viability and demonstrated reproducibility
- Reliable supply in cryopreserved format
More Data, More Publications, More Solutions
There is a broad range of peer-reviewed publications and CDI application protocols and applications notes to support your research goals including:
- Alzheimer’s disease research and drug discovery
Functional tau and β-amyloid toxicity pathways, long-term viability, and flexible product format enable high-throughput screening for novel drug candidates - Autism disease research
Demonstrated prefrontal cortex ionotropic glutamate receptors, GABAA receptor, and synaptic functionality drive interrogation of neurological diseases, such as autism - Infectious disease studies
Susceptibility to varicella zoster virus infection and ability to maintain viral latency and reactivation - Therapeutic potency testing
High sensitivity, dose-response, and biological responses to Clostridium botulinum neurotoxin (BoNT) aid replacement of the gold-standard mouse bioassay for BoNT potency testing - Neurotoxicity assessment
Compatibility with a wide range of high-content, high-throughput platforms enables assessment of pharmaceutical and environmental neurotoxicity
Components:
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Need Help With This Product?
Our specialists are here to help you find the best product for your application.
Our regular business hours are 9:00am to 5:00pm Central Time (USA)
Technical Docs
PROTOCOLS
Application Notes
Applying Transfection Technologies to Create Novel Screening Models - iCell Neural Products
Application Protocols
Assessing Neurite Outgrowth: Quantification with High Content Screening
Application Protocols
Immunofluorescent Labeling of iCell GABANeurons
Application Protocols
Plating into 1536-well Cell Culture Plates
Application Protocols
Silencing Gene Expression: siRNA Delivery by Transfection
Application Protocols
Using Liposome-mediated Transfection for Gene Delivery - iCell GABANeurons
Application Protocols
Tri-Culture of iCell Microglia with iCell Neuronal Cell Types and iCell Astrocytes
USER DOCS
User's Guides
iCell GABANeurons User's Guide
Datasheet
iCell GABANeurons Datasheet
Biosafety Document
iCell GABANeurons, 01279 Biosafety Document
Safety Data Sheet
iCell Cell-Based Products Safety Data Sheet - Asia
Safety Data Sheet
iCell Cell-Based Products Safety Data Sheet
Safety Data Sheet
iCell Neural Supplement A Safety Data Sheet
Safety Data Sheet
iCell Neural Supplement A Safety Data Sheet - Asia
Performance Data
Neurite Outgrowth Assay
Panels A and B show iCell GABANeurons, 01434 cultured in a 96-well cell culture plate in absence or presence of 50 ng/ml BDNF, respectively. The neurons were stained with calcein AM (green) and Hoechst 33342 (blue). Panels C and D show the segmentation masks generated from calcein AM and Hoechst 33342 fluorescent signals of panels A and B, respectively. Images were acquired using the ImageXpress Micro System, and segmentation masks were generated using the MetaXpress Software.
Figure 1: Representative Effect of BDNF on Neurite Outgrowth of iCell GABANeurons
Figure 2: Quantitative Effect of BDNF on Neurite Outgrowth of iCell GABANeurons
Quantitative Effect of BDNF on Neurite Outgrowth of iCell GABANeurons
Culturing iCell GABANeurons, 01434 in the presence of BDNF for 14 days produced the expected effect of enhancing neurite outgrowth. Data was acquired using the ImageXpress Micro System and quantified using the MetaXpress Software. Data are represented as mean values per number of neuronal cells (mean ± SEM, n = 6 wells).
Relevant Neuronal Markers and High Purity
iCell GABANeurons represent a highly pure population comprised primarily of GABAergic neurons with low levels of nestin (neuronal progenitor marker) as demonstrated by immunocytochemistry. These images show iCell GABANeurons, 01434: (A) ß-III tubulin (neuronal marker, green) and nestin (red), 7 days post-plating; (B) punctate staining pattern for the vesicular glutamate transporter 2 (vGLUT2, red) and vesicular GABA transporter (vGAT, green), 14 days post-plating, indicative of glutamatergic and GABAergic neuronal subtypes, respectively; and (C) gamma-aminobutyric acid (GABA, red) and microtubule associated protein 2 (MAP2, green), 14 days post-plating.
Figure 3: iCell GABANeurons Represent a Highly Pure Population of Human Neurons
Figure 4: iCell GABANeurons Exhibit Typical Neuronal Morphology
Expected GABAergic Neuron Morphology
These images show iCell GABANeurons, 01434 at days 1, 5, and 19 post-plating. The reanimated GABAergic neurons develop branched networks within 24 hours and remain viable and adherent for an extended period in culture (≥14 days).
Product Highlights
More Data, More Publications, More Solutions
There is a broad range of peer-reviewed publications and CDI application protocols and applications notes to support your research goals including:
- Alzheimer’s disease research and drug discovery Functional tau and β-amyloid toxicity pathways, long-term viability, and flexible product format enable high-throughput screening for novel drug candidates
- Autism disease research Demonstrated prefrontal cortex ionotropic glutamate receptors, GABAA receptor, and synaptic functionality drive interrogation of neurological diseases, such as autism
- Infectious disease studies Susceptibility to varicella zoster virus infection and ability to maintain viral latency and reactivation
- Therapeutic potency testing High sensitivity, dose-response, biological responses to Clostridium botulinum neurotoxin
- Neurotoxicity assessment Compatibility with a wide range of high-content, high-throughput platforms enables assessment of pharmaceutical and environmental neurotoxicity