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iCell DopaNeurons PD SNCA A53T HZ, 01279
Product Overview
SNCA A53T is one of the most highly penetrant and widely studied mutations linked to Parkinson’s disease (PD). SNCA encodes for the alpha-synuclein (α-syn) protein, which is predominantly expressed in the brain at presynaptic terminals. The A53T mutation renders α-syn more susceptible to aggregation and accumulation, which are hallmark indicators of PD pathology.
To generate a heterozygous (HZ) A53T allelic variant isogenic to iCell® DopaNeurons, nuclease-mediated SNP alteration of a healthy control iPS cell line was performed to introduce this site-specific mutation into the gene for SNCA. This genome-engineered iPS cell was then differentiated into human midbrain floorplate dopaminergic (DA) neurons according to protocols licensed and adapted from the Lorenz Studer lab (Memorial Sloan Kettering) and industrialized at CDI, to produce iCell DopaNeurons SNCA A53T HZ. Please also see the isogenic iCell DopaNeurons.
Best-in-Class Biology
iCell DopaNeurons SNCA A53T HZ exhibit the relevant biology and functionality to advance research and preclinical studies for devastating neurological disorders:
Components:
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Technical Docs
PROTOCOLS
Application Protocols
Immunofluorescent Labeling of iCell DopaNeurons
Application Protocols
Using Liposome-mediated Transfection for Gene Delivery - iCell DopaNeurons
USER DOCS
User's Guides
iCell DopaNeurons User's Guide
Biosafety Document
iCell DopaNeurons (A53T) Biosafety Document
Biosafety Document
iCell DopaNeurons Biosafety Document
Safety Data Sheet
iCell Neural Supplement B Safety Data Sheet
Safety Data Sheet
iCell Nervous System Supplement Safety Data Sheet
Safety Data Sheet
iCell Cell-Based Products Safety Data Sheet - Asia
Safety Data Sheet
iCell Cell-Based Products Safety Data Sheet
Safety Data Sheet
iCell Neural Base Medium 1 Safety Data Sheet
Performance Data
Comparison to Isogenic iCell DopaNeurons
qPCR analysis reveals decreased TH and DDC and increased COMT expression in A53T dopaminergic neurons suggesting that biosynthetic levels of DA in the SNCA A53T neurons should be decreased.
Comparison to Isogenic iCell DopaNeurons
Immunofluorescence staining shows detectable levels of alpha-synuclein in the control cells and more in the SNCA A53T DA neurons (data not quantified).
Comparison to Isogenic iCell DopaNeurons
Bioenergetic analysis of WT versus A53T cells indicates that the response to FCCP and the max respiratory capacity is greater in the mutant line. Data are normalized based on cell number.
Comparison to Isogenic iCell DopaNeurons
Both iCell DopaNeurons and iCell DopaNeurons SNCA A53T HZ display high activity and synchronous bursting on MEA; A53T cells have fewer bursts per minute, but the intensity of those bursts are ~5X greater.
Comparison to Isogenic iCell DopaNeurons
iCell SNCA A53T HZ DopaNeurons display spontaneous Ca2+ oscillations earlier in culture (e.g. day 7) with an amplitude and bursting pattern that is significantly different than the WT control.
Product Highlights
Easy to implement
iCell DopaNeurons are shipped cryopreserved with optimized media. Simply thaw and use.
Human cells
iCell DopaNeurons are floor plate-derived dopaminergic neurons from human iPS cells ensuring complete regional specification for full functionality.
Highly pure
iCell DopaNeurons are >80% tyrosine hydroxylase positive (THpos) midbrain dopaminergic neurons, enabling mechanistic studies of neurodegenerative diseases.
Homogenous and reproducible
iCell DopaNeurons are fully differentiated neurons, not precursors, providing biologically relevant and reproducible results.